Bacteriological and antibiotic resistance profile of germs isolated from pyocultures at the Biomedical Laboratory of the China-Guinea Friendship Hospital of Kipé in Conakry (Guinea)

Abstract

Introduction: The rapid evolution of bacterial multi-resistance to antibiotics is a worrying phenomenon in the world and particularly in Africa. Objective: The aim of this study was to describe the bacteriological and antibiotic resistance profile of germs isolated from pyocultures at the China-Guinea Friendship Hospital in Conakry (HASIGUI). Methods: This is a retrospective study  carried out at the HASIGUI Biomedical Laboratory from June 15^th, 2017 to December 22^th, 2021). Thus, 432 pus samples were analyzed. Bacterial identification and antibiograms were carried out using the Vitek2 Compact machine, and by the API and microdilution system (bioMérieux, France). Results: Male were predominant with a sex ratio (Male/Female) = 2.04, and the age group from 0 to 20 years was in the majority. The distribution according to professions showed that students were predominant. Of 432 pyocultures performed, 291 (67%) were positive. Bacterial identification showed that the species identified were Staphylococcus aureus (15.81%), Staphylococcus xylosus (6.53%), Staphylococcus lentus (4.81%) and Escherichia coli (4.47%). The majority of strains were susceptible to linezolid (82.47%), imipenem (71.51%), clindamycin (67.27%), fosfomycin (62.99%), levofloxacin (59. 62%), ciprofloxacin (52.66%). On the other hand, the majority of strains were resistant to benzylpenicillin (94.11%), cephalin (90.47%), cefixime (86.29%), cefuroxime (83.67%), ampicillin (77.17%), cefotaxime (73.94%), nalidixic acid (72.8%), cotrimoxazole (60.81%). Conclusion: All of these results show multi-resistance to antibiotics in bacteria isolated from pyocultures requiring treatment based on the results of antibiograms.